Synonyms:
OB Protein, Obesity Protein, OBS, Obesity factor, leptin (obesity homolog, mouse), ob
Description:
Background: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores. Product:
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered concentrated (1 mg/ml) solution in 0.0045 mM NaHCO3.
Purity:
>95% by SDS-PAGE and HPLC analyses.
Biological Activity:
Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor
Endotoxin Level:
<0.1 ng/�g
Amino Acid Sequence:
Ala-Val-Pro-Ile-Arg (First five N-terminal amino acids)
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile ddH2O to a concentration of ?0.1 mg/ml. The solution can then be further diluted in other aqueous buffers.
Storage/Stability:
Lyophilized protein is stable at room temperature for 3 weeks, but is preferably stored desiccated at -20℃C. After reconstitution, stable for 1 week at 2-4℃C. For long term storage, aliquot and store at -20℃C with a carrier protein (0.1%
