Recombinant Taq DNA Polymerase

Synonyms:

DNA polymerase I thermostable, Taq polymerase 1, EC 2.7.7.7

Cat. No.:

CSI10702 pdf (datasheet)

Quantity/Size:

10,000 U

Price:

$560.00 BUY

Description:

Taq DNA Polymerase is a thermostable enzyme of approximately 94 kDa isolated from the bacterium Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5´~3´ direction in the presence of magnesium and also possesses 5´~3´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.

Data PDF:

CSI10700

Source:

E. coli

Molecular Weight:

94 kDa

Formulation:

Taq DNA Polymerase solution in 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 5 mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20.

Purity:

> 95% by SDS-PAGE analysis

Biological Activity:

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50 mM Tris-HCl (pH 9.0 at 25°C), 50 mM NaCl, 5 mM MgCl2, 200 μm each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10 µg activated calf thymus DNA, 0.1mg/ml BSA in a final volume of 50 ul.

Storage/Stability:

Stable for 5 days at 2-8°C, for longer period of time store at -20°C to -80°C.

Application notes:

PCR, 3´ A-tailing of blunt ends, compatible with Vectors.