Basic research looks to tackle today’s most challenging problems, but it also gives us a glimpse into the future’s most elegant solutions whether they are slight tweaks or revolutionary paradigm changes. There are many exciting areas of research emerging as the application for recombinant proteins continues to grow. Though, in turn they are becoming more specific in their uses and the composition of the cell culture medium has become even more scrutinized. Animal-free recombinant proteins are one product that has begun to play an important role in a wide array of experimental procedures.
The development of animal-free recombinant proteins eliminates the risk of contamination that has been associated with animal and human serum-derived media. It goes beyond serum-free media, which while not supplemented with serum may contain discrete proteins or bulk proteins. Animal-free contains no components of animal or human origin. It is manufactured in a completely animal-free area with dedicated labware, fermenters, filters, lyophilizers, to guarantee the absence of even trace amounts of animal proteins or mammalian pathogens like Mycoplasma or Spiroplasma which slow growth rate, productivity, and density.
Researchers utilize animal-free recombinant proteins in their work for multiple reasons and capacities. The high purity (>95%) and low endotoxin levels (<1.0 EU per ug protein via LAL) not only reduces multiple experimental variables, but works as an excellent experimental control. They are regularly used in antibody preparation, enzyme activity assay, in vivo evaluation, in vitro diagnosis, ex vivo preclinical research, pilot stage bio production, and even stem cell research. As stem cells have this unique ability to self-renew or to differentiate into various cell types, they require the use of special stem cell culture media because suboptimal media may change the differentiation potential of stem cells.
As the industry moves towards high quality, defined, animal-free cell culture products, not only do bio-manufacturers eliminate the risk of contamination that has been associated with animal and human serum derived media, but also the purity and simplicity of the culture media presents opportunities from a cost-reduction perspective in purification and downstream processing.
Cell Sciences is proud to be working to attain research goals, advance life sciences research, and support clinical breakthroughs by offering animal-free recombinant protein product lines.
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