First described over two decades ago, the Interleukin-17 (IL-17) cytokine family are strong inducers of inflammation and play a pivotal role in host defense against microbial infection. Yet in stark contrast to this essential protective function, they are implicated in the pathogenesis of various chronic inflammatory and autoimmune diseases and have also demonstrated a contributory role in cancer. Now recognized as important therapeutic targets, IL-17 cytokines are widely-studied across a diversity of research areas. High-quality IL-17 reagents are fundamental to support this work.
Discovery of IL-17 cytokines
The gene encoding IL-17A was first discovered in 1993. Then termed CTLA-8, and subsequently renamed as IL-17 and then IL-17A, the encoded protein represents the first of six IL-17 family members to be described. The IL-17 cytokine family is now known to consist of IL-17A through to IL-17F.
Following its discovery, IL-17A was linked to inflammation by the demonstration that it could stimulate epithelial, endothelial, and fibroblastic cells to secrete potent inflammatory cytokines such as IL-6, IL-8, and GM-CSF. It was also shown to drive the maturation of CD34+ hematopoietic progenitors into neutrophils, key mediators of inflammation which perform a crucial role in the innate immune response.
Role in the host immune response
IL-17 cytokines are produced by T-helper 17 (Th17) cells. These originate from naïve CD4+ T-cells following host infection with a pathogen such as a bacterium or fungus. It is important to note that IL-17 production is not exclusive to Th17 cells, since CD8+ T-cells and γδ T cells (innate immune cells that play important roles at the mucosal barrier) also produce IL-17 cytokines. Despite their low abundance, γδ T cells are often the main source of IL-17A in various inflammatory disease models.
IL-17 cytokines exert their role in host defense primarily through the induction of anti-microbial molecules and mediation of neutrophil recruitment at the site of infection. This is achieved through binding to IL-17 receptors (IL-17R). Found on a wide variety of cell types, the IL-17 receptor family consists of five variants (IL-17RA through IL-17RE). These perform distinct signaling functions.
Role in disease
IL-17 cytokines are known to contribute to the pathogenesis of many inflammatory and autoimmune disease conditions. These include psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn’s disease. The IL-17 cytokine family also has a prominent role in promoting carcinogenesis, tumor metastasis and resistance to chemotherapy, making IL-17 cytokines attractive targets for oncology research.
The first therapeutic antibody against IL-17A – Secukinumab for the treatment of psoriasis – was approved in 2015. However, despite sharing similar immune characteristics, inflammatory bowel disease has not been successfully treated with this drug. This highlights the complexity of IL-17 cytokine signaling and indicates the need for ongoing IL-17 research. With many other therapeutic agents targeting IL-17 cytokines currently under development, trusted reagents to support this work are essential.
Supporting IL-17 research
Offering a wide range of high-quality reagents, Cell Sciences is your partner of choice for IL-17 research. Included within our IL-17 product portfolio are:
- ELISA kits
- ELISpot kits
- Antibodies (including matched antibody pairs)
- Recombinant cytokines
Standard curve generated using human IL-17A ELISA kit (CDK043). One of our most popular IL-17 products, CDK043 recognizes both native and recombinant IL-17A with sensitivity <10 pg/mL. Calibrated against the International Reference Standard NIBSC 01/420 IL-17A to assure product quality, CDK043 demonstrates no cross-reactivity with other cytokines and delivers robust and reproducible data in under 4 hours.
Other popular IL-17 products include:
ELISA & ELISpot Kits
- Human IL-17F ELISA kit (CDK044)
- Human IL-17A/IL-17F ELISA kit (CDK045)
- Mouse IL-17A ELISA kit (CDK070)
- Human IL-17 ELISA kit (CKH157)
- Mouse IL-17A Total Antigen ELISA kit (CS451)
- Human IL-17A ELISpot kit (CDK117)
- Human IL-17F ELISpot kit (CDK119)
- Neutralizing anti-Human IL-17A, clone B-B51 (CDM114)
- Neutralizing anti-Human IL-17F clone B-F60 (CDM288)
- Rabbit anti-Human IL-17 pAb (PA0692)
- Rabbit anti-Human IL-17E pAb (PA0694)
- Matched Antibody Pair for Human IL-17A ELISA (CDK089)
- Recombinant Human IL-17A (CRI231)
- Recombinant Human IL-17AF Heterodimer (CS307)
- Recombinant Human IL-25/IL-17E (CRI196)
- Recombinant Human IL-17F (CRI197)
- Recombinant Human IL-17B (CRI198B)
To learn more about our human IL-17A ELISA kit, or any of our IL-17 products, contact us today.