Lipopolysaccharide-Binding Protein (LBP) as an Indicator of Disease States in Multiple Species
Amanda Flies, Ph.D. and Thomas H. Ermak, Ph.D.
LBP binds multimers of LPS in the blood or other bodily fluids and transfers LPS monomers to CD14 on the surface of an innate immune cell. LPS is detected at the cell’s surface by a protein complex involving CD14, MD2 and TLR4, which signals through its intracellular domain. Recognition of bacterial products such as LPS may activate innate immune cells to produce inflammatory mediators.
Lipopolysaccharide binding protein (LBP) is a 58-kD glycoprotein involved in the acute-phase immunologic response to Gram-negative bacterial infections. It binds with high affinity to the lipid A portion of lipopolysaccharide (LPS), which Gram-negative bacteria express on their outer membrane. The presence of LBP in blood and bodily fluids is an indicator of bacterial infection, and as such is a useful marker of a number of disease states in humans, cows, mice and other animals.
As a method of detecting this important acute-phase protein, ELISA (enzyme-linked immunosorbent assay) offers specificity and reliable quantitation of the protein levels in samples such as blood, serum, lavage fluid, or milk. The Cell Sciences® Multispecies LBP ELISA kit maintains specificity for human LBP, while also providing flexibility to assess a broad range of LBP homologs in other species.
LBP Reagents offered by Cell Sciences
CKH113 Human LBP Multispecies Reactive ELISA Kit
CKM043 Mouse LBP ELISA Kit
CML002 Mouse Anti-Human LBP Clone biG 42 mAb†
CML003 Mouse Anti-Human LBP Clone biG 48 mAb†
CML004 Mouse Anti-Human LBP Clone biG 412 mAb*
CML007 Mouse Anti-Human LBP Clone biG 43 mAb†
CPC402A Rabbit Anti-Human LBP pAb
CML001 Mouse Anti-Mouse LBP Clone biG 33 mAb*
CML005 Mouse Anti-Mouse LBP Clone biG 35 mAb†
CPC105 Rabbit Anti-Mouse LBP pAb
CRL701 Recombinant Human LBP
CRL700 Recombinant Mouse LBP
* Inhibits binding to CD14 † Does not inhibit binding to CD14