Human Testis Tissue Lysate

Molecule:

Testis Tissue

Species:

Human

Cat. No.:

PX470A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$175.00 BUY

Description:

Human testis tissue lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCI, pH 7.4 + 1% Triton X-100 + 0.2% sodium deoxycholate + 0.2% sodium dodecylsulfate (SDS) + 1 mM sodium ethylenediaminetetraacetate + 1 mM phenylmethylsulfonyl fluoride + 5 µg/ml aprotinin + 5 µg/ml leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl, pH 6.8 + 12.5% glycerol + 1% sodium dodecylsulfate + 0.01% bromophenol blue) containing 5% beta-mercaptoethanol

Concentration:

2.0 mg/ml

Data PDF:

PX470

Source:

Human testis

Formulation:

Testis tissue lysate is supplied in 1 x SDS sample buffer containing 5% betamercaptoethanol.

Storage/Stability:

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year.

Application notes:

Centrifuge vial before use. The tissue lysate is ready to load on SDS-PAGE for Western blot. 10-20 μg per lane is recommended for mini gel.

Application:

WB